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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: The Neuroprotective Effects of Exosomes Derived from TSG101-Overexpressing Human Neural Stem Cells in a Stroke Model
doi: 10.3390/ijms23179532
Figure Lengend Snippet: Neuroprotective and anti-inflammatory effects of exosomes in N2A cells subjected to oxygen–glucose deprivation (OGD). ( A ) Neuroprotective effects of exosomes under 3 h OGD exposure in the lactate dehydrogenase (LDH) assay; ( B – F ) real-time PCR analysis of mRNA of nuclear factor (NF)-κB ( B ), tumor necrosis factor (TNF)-α ( C ), interleukin (IL)-6 ( D ), inducible nitric oxide synthase (iNOS) ( E ), and cyclooxygenase 2 (COX2) ( F ) normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH); ( G , H ) Western blot analysis of sirutin 1 (SIRT1), histone deacetylase (HDAC), and forkhead box O-3 (FOXO3) showing representative bands ( G ) and band densities normalized to actin ( H ). Densitometric analysis of the Western blot was performed using ImageJ. n = 3 per treatment group. * Significantly different from normal control ( p < 0.05). # Significantly different from vehicle control ( p < 0.05).
Article Snippet: LDH content was determined using a commercial
Techniques: Lactate Dehydrogenase Assay, Real-time Polymerase Chain Reaction, Western Blot, Histone Deacetylase Assay